产品详情
产品名称PKM1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IF
种属反应性Hu Ms
特异性The antibody detects endogenous levels of total PKM1 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 399~403(V-R-A-S-S) derived from Human PKM1.
基因/蛋白名称PKM1
别名PKM; PK3; OIP3; PK2;
数据库入口号Swiss-Prot: P14618
NCBI Protein: NP_872270.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:1000
背景
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.
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et al,Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation. In Biomedicines on 2022 May 27 by Abu Jubayer Hossain, Rokibul Islam, et al..PMID:35740278,
, (2022),
PMID: 35740278
et al,Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition. In Biomolecules on 2022 Aug 6 by Seong Ho Kim, Ji Hun Wi, et al..PMID:36008976,
, (2022),
PMID: 36008976
et al,A novel miR-206/hnRNPA1/PKM2 axis reshapes the Warburg effect to suppress colon cancer growth. In Biochem Biophys Res Commun on 2020 Oct 22 by Rong Fu, Peng Yang, et al..PMID:32800545,
, (2020),
PMID: 32800545
et al,Phosphorylation of pyruvate kinase M2 and lactate dehydrogenase A by fibroblast growth factor receptor 1 in benign and malignant thyroid tissue.In BMC Cancer on 2015 Mar 18 by Paul Kachel , Bogusz Trojanowicz et al..PMID:25880801,
, (2015),
PMID: 25880801
et al,Pyruvate kinase M2 accelerates pro-inflammatory cytokine secretion and cell proliferation induced by lipopolysaccharide in colorectal cancer. In Cell Signal on 2015 Jul by Peng Yang, Zongwei Li, et al..PMID:25778902,
, (2015),
PMID: 25778902
et al,Regulatory circuit of PKM2/NF-魏B/miR-148a/152-modulated tumor angiogenesis and cancer progression. In Oncogene on 2015 Oct by
Q Xu, L-Z Liu,et al..PMID:25703326,
, (2015),
PMID: 25703326
et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223,
, (2014),
PMID: 24316223
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et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762,
, (2014),
PMID: 25412762
et al,Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling.In Cell Signal on 2014 Sep by Peng Yang, Zongwei Li et al..PMID:24686087,
, (2014),
PMID: 24686087
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by
Weiwei Yang, Yan Xia, et al..PMID: 23123196,
, (2012),
PMID: 23123196
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803,
, (2012),
PMID: 22901803
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