免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > ATF2(Phospho-Ser112 or 94) Antibody

ATF2(Phospho-Ser112 or 94) Antibody#11033

ATF2(Phospho-Ser112 or 94) Antibody
ATF2(Phospho-Ser112 or 94) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称ATF2(Phospho-Ser112 or 94) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of ATF-2 only when phosphorylated at serine 112 or 94.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 112 or 94 (D-L-S(p)-P-L) derived from Human ATF2.

基因/蛋白名称ATF2

修饰Phospho

别名CREB2; CREBP1;

数据库入口号Swiss-Prot: P15336
NCBI Protein: NP_001871.2

UniprotP15336

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 65-75 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Huvec cells untreated or treated with Anisomycin using ATF2(Phospho-Ser112 or 94) Antibody #11033.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser112 or 94) Antibody #11033(left) or the same antibody preincubated with blocking peptide(right).
背景
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.

如果您使用该产品11033发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com