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位置: 首页 > 磷酸化抗体 > Myc(Phospho-Thr358) Antibody

Myc(Phospho-Thr358) Antibody#11035

Myc(Phospho-Thr358) Antibody
Myc(Phospho-Thr358) Antibody
Myc(Phospho-Thr358) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Myc(Phospho-Thr358) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of Myc only when phosphorylated at threonine 358.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 358 (R-R-T(p)-H-N) derived from Human Myc.

基因/蛋白名称Myc

修饰Phospho

别名c-myc

数据库入口号Swiss-Prot: P01106
NCBI Protein: NP_002458.2

UniprotP01106

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HT29 cells untreated(lane 1) or treated with UV(lane 2) using Myc(Phospho-Thr358) Antibody #11035.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Phospho-Thr358) Antibody #11035(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Myc(Phospho-Thr358) Antibody #11035.
背景
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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