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SPARC Antibody#21633

SPARC Antibody
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产品总价:
产品详情

产品名称SPARC Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of total SPARC protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.

基因/蛋白名称SPARC

别名ON; BM-40;

数据库入口号Swiss-Prot: P09486
NCBI Protein: NP_003109.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 42kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from T98G cells using SPARC Antibody #21633
背景
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com