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MEK1/2 Antibody#21428

MEK1/2 Antibody
是否有货: 有货
产品总价:
产品详情

产品名称MEK1/2 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total MEK1/2 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.235-239(L-Q-G-T-H) derived from Human MEK1/2.

基因/蛋白名称MEK1/2

别名MEK1; MKK1; MAPKK1; PRKMK1;

数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Hela and JK cells using MEK1/2 Antibody #21428
背景
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Seger R., Seger D., Lozeman F.J., Ahn N.G., Graves L.M., Campbell J.S., Ericsson L., Harrylock M., Jensen A.M., Krebs E.G.J. Biol. Chem. 267:25628-25631(1992)
Mukherjee S., Keitany G., Li Y., Wang Y., Ball H.L., Goldsmith E.J., Orth K.Science 312:1211-1214(2006)

如果您使用该产品21428发表了文章,请通知我们,让我们可以引用您的文献。

et al,7?dehydrocholesterol suppresses melanoma cell proliferation and invasion via Akt1/NF?kB signaling,In Oncol Lett on 2020 Dec by Jia Liu, Feiliang Zhong, et al..PMID: 33193858, , (2020),
PMID: 33193858
et al,Integrin 伪6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy.In Sci Rep on 2016 Sep 14 by Ting Hu, Rui Zhou et al..PMID:27624978 , , (2016),
PMID: 27624978
et al,MicroRNA锟?46b, a sensitive indicator of mesenchymal stem cell repair of acute renal injury.In Stem Cells Transl Med on 2016 Oct by Yuan Zhu, Jing Yu et al..PMID:27400799, , (2016),
PMID: 27400799
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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