产品详情
产品名称LIN28B Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB
种属反应性Hu
特异性The antibody detects endogenous levels of total LIN28B protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
基因/蛋白名称LIN28B
别名CSDD2; FLJ16517;
数据库入口号Swiss-Prot: Q6ZN17
NCBI Protein: NP_001004317.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 21 32kd
Western blotting: 1:500~1:1000
背景
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. "Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma."
Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) "Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA."
Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) "TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation."
Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009)
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et al,The KRAS/Lin28B axis maintains stemness of pancreatic cancer cells via the let锟?i/TET3 pathway. In Mol Oncol on 2021 Jan by Yawen Liu, Dawei Wang,et al..PMID:33107691,
, (2021),
PMID: 33107691
et al,Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics.In Mol Med Rep on 2020 May;21 by Wang B, Wang L, et al..PMID: 32186775,
, (2020),
PMID: 32186775
et al,Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway.In Mol Med Rep.On 2016 Oct by Gu H, Ji R et al..PMID:27513187,
, (2016),
PMID: 27513187
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