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位置: 首页 > 磷酸化抗体 > STAT3(Phospho-Tyr705) Antibody

STAT3(Phospho-Tyr705) Antibody#11045

STAT3(Phospho-Tyr705) Antibody
STAT3(Phospho-Tyr705) Antibody
STAT3(Phospho-Tyr705) Antibody
STAT3(Phospho-Tyr705) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称STAT3(Phospho-Tyr705) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of STAT3 only when phosphorylated at tyrosine 705.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 705 (A-P-Y(p)-L-K) derived from Human STAT3.

基因/蛋白名称STAT3

修饰Phospho

别名APRF; Acute-phase response factor; HIES

数据库入口号Swiss-Prot: P40763
NCBI Protein: NP_003141.2

UniprotP40763

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 88kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Hela cells untreated or treated with IFN a using STAT3(Phospho-Tyr705) Antibody #11045.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody #11045 (left) or the same antibody preincubated with blocking peptide #51045 (right).
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody #11045.
Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody #11045.
背景

Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Welsh, et al. (1996) Trends Cell Biol. 6: 274-279.
Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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