产品详情
产品名称PKM2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IF
种属反应性Hu
特异性The antibody detects endogenous levels of total PKM2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.405~409(T-S-D-P-T) derived from Human PKM2.
基因/蛋白名称PKM2
别名PKM; PK3; OIP3; PK2;
数据库入口号Swiss-Prot: P14618-1
NCBI Protein: NP_872270.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 60kd
Western blotting: 1:500~1:1000
背景
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.
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et al,Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition. In Biomolecules on 2022 Aug 6 by Seong Ho Kim, Ji Hun Wi, et al..PMID:36008976,
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et al,PK-M2-mediated metabolic changes In breast cancer cells Induced by ionizIng radiation. In Breast Cancer Res Treat on 2019 Nov by Zhang L, Bailleul J, et al..PMID: 31372790 ,
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et al,IGF-1-mediated PKM2/尾-catenin/miR-152 Regulatory Circuit in Breast Cancer.In Sci Rep on 2017 Nov 21 by Yi-Yang Wen, Wei-Tao Liu,et al..PMID: 29162853,
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et al,Nuclear PKM2 expression, an independent risk factor for ER after curative resection of hepatocellular carcinoma.In Biomed Pharmacother.On 2016 Dec by Fan F, Wu H et al..PMID: 27894667 ,
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et al,EGFR phosphorylates FAM129B to promote Ras activation.In Proc Natl Acad Sci USA on 2016 Jan 19 by Haitao Ji, Jong-Ho Lee et al..PMID:26721396,
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et al,PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis.In Nat Commun on 2016 Aug 3 by Ji Liang , Ruixiu Cao et al..PMID: 27485204,
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PMID: 27485204
et al,Relationship between 18-F-fluoro-deoxy-d-glucose uptake and expression of glucose transporter 1 and pyruvate kinase M2 in intrahepatic cholangiocarcinoma.In Dig Liver Dis
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et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223,
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PMID: 24316223
et al,PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells.In Nat Commun on 2014 Nov 21 by Yuhui Jiang , Yugang Wang et al..PMID: 25412762,
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PMID: 25412762
et al,Insulin regulates glucose consumption and lactate production through reactive oxygen species and pyruvate kinase M2. In Oxid Med Cell Longev on 2014 by Qi Li, Xue Liu, et al..PMID:24895527,
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PMID: 24895527
et al,Nuclear PKM2 Regulates the Warburg Effect.In Cell Cycle on 2013 Oct 1 by Weiwei Yang, Zhimin Lu,et al..PMID:24013426,
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PMID: 24013426
et al,ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.In Nat Cell Biol on 2012 Dec by
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et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803,
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