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位置: 首页 > 磷酸化抗体 > Histone H3(Phospho-Thr11) Antibody

Histone H3(Phospho-Thr11) Antibody#11577

Histone H3(Phospho-Thr11) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Histone H3(Phospho-Thr11) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Histone H3 only when phosphorylated at threonine 11.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 11(K-S-T(p)-G-G) derived from Human Histone H3.

基因/蛋白名称Histone H3

修饰Phospho

别名H3/a H3/m H3.3A; H3/c H3/o H3F3B; H3/d; H3/f; H3/h

数据库入口号Swiss-Prot: P68431, Q71DI3, P84243
NCBI Protein: NP_003521.2 NP_066403.2 NP_002098.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 17kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from U87 cells untreated or treated with EGF using Histone H3(Phospho-Thr11) Antibody #11577.
背景
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Preuss U., Landsberg G., Scheidtmann K.H.
Nucleic Acids Res. 31:878-885(2003)
Shimada M., Niida H., Zineldeen D.H., Tagami H., Tanaka M., Saito H., Nakanishi M.Cell 132:221-232(2008)
Metzger E., Yin N., Wissmann M., Kunowska N., Fischer K., Friedrichs N., Patnaik D., Higgins J.M., Potier N., Scheidtmann K.H., Buettner R., Schule R.Nat. Cell Biol. 10:53-60(2008)

如果您使用该产品11577发表了文章,请通知我们,让我们可以引用您的文献。

et al,RNF8 Mediates Histone H3 Ubiquitylation and Promotes Glycolysis and Tumorigenesis.In J Exp Med on 2017 Jun 5 by Yan Xia , Weiwei Yang,et al..PMID: 28507061, , (2017),
PMID: 28507061
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803, , (2012),
PMID: 22901803
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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