产品详情
产品名称CREB(Phospho-Ser133) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of CREB only when phosphorylated at serine 133.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 133 (R-P-S(p)-Y-R) derived from Human CREB.
基因/蛋白名称CREB
修饰Phospho
别名CREB-1; CREB1;
数据库入口号Swiss-Prot: P16220
NCBI Protein: NP _004370.1
UniprotP16220
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
背景
CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .
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Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
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