产品详情
产品名称FAK(Phospho-Tyr861) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Hu
特异性The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from Human FAK.
基因/蛋白名称FAK
修饰Phospho
别名FADK 1; FAK1; PTK2
数据库入口号Swiss-Prot: Q05397
NCBI Protein: NP _005598.3
UniprotQ05397
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 125kd
Western blotting: 1:500~1:1000
背景
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
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Masahiko Kanehira, Toshiaki Kikuchi, Shinya Ohkouch et al,Targeting Lysophosphatidic Acid Signaling Retards Culture-Associated Senescence of Human Marrow Stromal Cells. ,
PLoS ONE, 7(2): e32185. doi:10.1371/journal.pone.0032185 (2012),
PMID: 22359668
et al,Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells.In PLoS One on 2012
by Kanehira M, Kikuchi T, et al..PMID:22359668,
, (2012),
PMID: 22359668
aiyang Sheng, Bo Song, Zhenhuan Zheng et al,Abnormal cleavage of APP impairs its functions in cell adhesion and migration,
Neuroscience Letters, 450, 327篓C33 (2009),
PMID: 19056463
et al,Abnormal cleavage of APP impairs its functions in cell adhesion and migration.In Neurosci Lett on 2009 Feb 6 by Baiyang Sheng, Bo Song,et al..PMID:19056463,
, (2009),
PMID: 19056463
et al,Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration. In J Biomater Appl on 2009 Sep by
Zhenhuan Zheng, Yujun Wei, et al..PMID: 18987023,
, (2009),
PMID: 18987023
et al,Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells. In Eur J Pharmacol 2009 Jan 5 by Liang Wu, Lei Zhu, et al..PMID:
19000670,
, (2009),
PMID: 19000670
Z Zheng, Y Wei et al,Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration.,
Journal of Biomaterials Applications, 24:209-229 (2008),
PMID: 18987023
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Liang Wu, Lei Zhu, Wei-Hao Shi et al,Zoledronate inhibits the proliferation,adhesion and migration of vascular smooth muscle cells.,
European Journal of Pharmacology, 602, 124篓C131 (2008),
PMID: 19000670
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