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位置: 首页 > 磷酸化抗体 > Ezrin(Phospho-Tyr353) Antibody

Ezrin(Phospho-Tyr353) Antibody#11063

Ezrin(Phospho-Tyr353) Antibody
Ezrin(Phospho-Tyr353) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Ezrin(Phospho-Tyr353) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from Human EZRIN.

基因/蛋白名称Ezrin

修饰Phospho

别名Cytovillin; EZRI; VIL2; Villin 2; p81

数据库入口号Swiss-Prot: P15311
NCBI Protein: NP_001104547.1

UniprotP15311

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 81kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Western blot analysis of extracts from 293 cells untreated or treated with EGF using Ezrin(Phospho-Tyr353) Antibody #11063.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin(Phospho-Tyr353) Antibody #11063(left) or the same antibody preincubated with blocking peptide(right).
背景

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305.
Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
 

如果您使用该产品11063发表了文章,请通知我们,让我们可以引用您的文献。

et al,Prognostic implications of ezrin and phosphorylated ezrin expression in non-small cell lung cancer.In BMC Cancer on 2014 Mar 15 by Tiefeng Jin, Jingchun Jin et al ..PMID: 24629131, , (2014),
PMID: 24629131
et al,Differential ezrin and phosphorylated ezrin expression profiles between pancreatic intraepithelial neoplasia, intraductal papillary mucinous neoplasm, and invasive ductal carcinoma of the pancreas. In Hum Pathol on 2013 Aug by Yasunori Oda, Shinichi Aishima, et al..PMID: 23465281, , (2013),
PMID: 23465281
Yasunori Oda MD, Shinichi Aishima PhD, Katsuya Morimatsu PhD et al,Differential ezrin and phosphorylated ezrin expression profiles between pancreatic intraepithelial neoplasia, intraductal papillary mucinous neoplasm, and invasive ductal carcinoma of the pancreas., Human Pathology, 44(8):1487篓C1498 (2012),
PMID: 23465281
Yazhou Cui, Tianliang Li, Denglu Zhang et al,Expression of Ezrin and Phosphorylated Ezrin (pEzrin) in Pancreatic Ductal Adenocarcinoma., Cancer Investigation, 28(3) 242-247 (2010),
PMID: 20158339
et al,Expression of Ezrin and Phosphorylated Ezrin (pEzrin) in Pancreatic Ductal Adenocarcinoma.In Cancer Invest on 2010 Mar by Cui Y1, Li T,et al..PMID:20158339, , (2010),
PMID: 20158339
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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