产品详情
产品名称Estrogen Receptor-a(Phospho-Ser106) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of Estrogen
Receptor-a only when phosphorylated at serine 106.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine106 (S-P- S(p)-P-L) derived from Human Estrogen Receptor-a.
基因/蛋白名称Estrogen Receptor-a
标记Unconjugated
别名ER; ESR; ESR1; ESTR; ESTRA
数据库入口号Swiss-Prot: P03372
NCBI Protein: NP_000116.2
Uniprot
P03372
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 66kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF7 cells untreated or treated with EGF using Estrogen Receptor-a(Phospho-Ser106) Antibody #11071.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser106) Antibody #11071(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser106) Antibody #11071.
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.
Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
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et al,Astragalus root induces ovarian β?oxidation and suppresses estrogen?dependent uterine proliferation.In Mol Med Rep. On 2018 Dec Orkhon B1, Kobayashi K et al..PMID: 30272268
, (2018),
PMID:
30272268
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