产品详情
产品名称ADD1(Phospho-Ser726) Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of ADD1only when phosphorylated at serine 726.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
基因/蛋白名称ADD1
标记Unconjugated
别名ADDA; Erythrocyte adducin alpha subunit;
数据库入口号Swiss-Prot: P35611
NCBI Protein: NP_001110.2
Uniprot
P35611
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody #11182 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody #11182(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody #11182.
Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12
Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51
Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70
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et al,Adducin‐1 Facilitates Influenza Virus Endosomal Trafficking and Uncoating by Regulating Branched Actin Dynamics and Myosin IIB Activity
, (2025),
PMID:
40472230
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