产品详情
产品名称MEK1(Phospho-Thr291) Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of threonine 291 (P-R-T(p)-P-G derived from Human MEK1.
基因/蛋白名称MEK1
标记Unconjugated
别名ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1
Uniprot
Q02750
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells untreated or treated with PMA using MEK1(Phospho-Thr291) Antibody #11294.
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Kevin D. Burroughs, et,al. (2003) Mol. Cancer Res ; 1: 312.
Michael J. Piatelli, et,al. (2002) J. Biol. Chem ; 277: 12144 - 12150.
Margaret M. Morgan, et,al. (2001) J. Immunol ; 167: 5708.
Herbert Schramek, et,al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661.
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et al,CD40-expressing plasmid induces anti-CD40 antibody and enhances immune responses to DNA vaccination.
, (2010),
PMID:
19950201
et al,Magnesium deprivation inhibits a MEK CERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells.
, (2010),
PMID:
20338184
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