产品详情
产品名称TAK1 (Phospho-Thr184) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.
免疫原类型peptide
免疫原描述Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1.
基因/蛋白名称TAK1
标记Unconjugated
别名EC 2.7.11.25; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; Transforming growth factor-beta-activated kinase 1; kinase TAK1
数据库入口号Swiss-Prot#:O43318;
NCBI Gene#:6885
Uniprot
O43318
计算分子量67 kDa
实际分子量75 kDa
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
All lanes : TAK1 (Phospho-Thr184) Antibody at 1/500 dilution Lane 1 : HepG2 whole cell lysates Lane 2 : HepG2 treated with 20ng/ml TNFa for 5 minutes whole cell lysates Lane 3 : 293T whole cell lysates Lane 4 : 293T treated with 20ng/ml TNFa for 5 minutes whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 67 kDa Observed band size: 75 kDa Exposure time: 9 seconds
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue using TAK1 (Phospho-Thr184) Antibody #12153.
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.
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et al,PGC-1β suppresses saturated fatty acid-induced macrophage inflammation by inhibiting TAK1 activation. In IUBMB Life on 2016 Feb by Hongen Chen, Yan Liu, et al..PMID: 26748475
, (2016),
PMID:
26748475
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