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位置: 首页 > 磷酸化抗体 > Estrogen Receptor-β (Phospho-Ser87) Antibody

Estrogen Receptor-β (Phospho-Ser87) Antibody#12568

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产品详情

产品名称Estrogen Receptor-β (Phospho-Ser87) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Human

特异性Estrogen Receptor-β (Phospho-Ser87) Antibody detects endogenous levels of Estrogen Receptor-β only when phosphorylated at Ser87

免疫原类型Peptide

免疫原描述A synthesized peptide derived from human Estrogen Receptor-β (Phospho-Ser87)

基因/蛋白名称Estrogen Receptor-β

标记Unconjugated

别名ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

数据库入口号Swiss-Prot#: Q92731
NCBI Gene ID: 2100

Uniprot Q92731

计算分子量59kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

Target种属human

应用详情

Western blotting: 1:1000

All lanes : Estrogen Receptor-β (Phospho-Ser87) Antibody at 1/500 dilution
Lane 1 : K562 whole cell lysates
Lane 2 : K562 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lane 4 : MCF7 treated with 100nM Estradiol for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 7 seconds

如果您使用该产品12568发表了文章,请通知我们,让我们可以引用您的文献。

et al,Diselenide Nanogels Modulate Mitochondrial Function and Mitigate Oxidative Stress in Cardiomyocytes for Enhanced Cardiac Repair , (2025),
PMID: 40028900

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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