Yes
All lanes : TrkB(Phospho-Y817) Rabbit mAb at 1/1k dilution
Lane 1 : SH-SY5Y whole cell lysates
Lane 2 : Mouse brain tissue lysates
Lane 3 : Rat brain tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 145 kDa
Observed band size: 140 kDa
Exposure time: 12 seconds
Formalin-fixed;paraffin-embedded human kidney tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human pancreas tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded mouse pancreas tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TrkB(Phospho-Y817) antibody (13384)
ICC/IF staining of TrkB(Phospho-Y817) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13384 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
The cells were incubated 30 minutes at room temperature with phalloidin tritc .
Nuclei were counterstained with DAPI.
