Yes
All lanes: MouseK1(Phospho-S376) Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates
Lane 2 : 293 treated with 200nM TPA for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 10 seconds
Formalin-fixed;paraffin-embedded mouse bladder tissue stained for MouseK1(Phospho-S376) using 13389 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human liver cancer tissue stained for MouseK1(Phospho-phospho S376) using 13389 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence MouseK1(Phospho- S376) antibody (13389)
ICC/IF staining of MouseK1(Phospho- S376) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13389 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence MouseK1(Phospho-S376) antibody (13389)
ICC/IF staining of MouseK1(Phospho-S376) in PC-3M cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13389 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
