Yes
All lanes : c-Jun(Phospho-T91) Rabbit mAb at 1/500 dilution
Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 treated with 250nM Anisomycin for 30 minutes whole cell lysates
Lane 3 : A549 whole cell lysates
Lane 4 : A549 treated with 250nM Anisomycin for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa
Exposure time: 15 seconds
Formalin-fixed;paraffin-embedded human breast carcinoma tissue stained for c-Jun (Phospho-T91) using 13420 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence cJun (Phospho-T91) antibody (13420)
ICC/IF staining of cJun(Phospho-T91) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13420 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
