Yes
All lanes : Creb (Phospho-S133) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : HeLa treated with 10 µM Forskolin for 30min whole cell lysatesSecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 35 kDa Observed band size: 46 kDaExposure time: 5 seconds
Formalin-fixed;paraffin-embedded human spleen tissue stained for Creb (Phospho-S133) using 13435 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded mouse colon tissue stained for Creb (Phospho-S133) using 13435 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human colon cancer tissue stained for Creb (Phospho-S133) using 13435 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Creb (Phospho-S133) antibody (13435)
ICC/IF staining of Creb (Phospho-S133) in SHSY5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13435 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Creb (Phospho-S133) antibody (13435)
ICC/IF staining of Creb (Phospho-S133) in HUVEC cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13435 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
