产品详情
产品名称ATF-2 (Phospho-Thr69) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB;IHC;IF;IP;ELISA
种属反应性Human;Mouse;Rat
特异性Phospho-ATF-2 (T69) Polyclonal Antibody detects endogenous levels of ATF-2 protein only when phosphorylated at T69.
免疫原描述The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Thr69 or 51. AA range:36-85
标记Unconjugated
别名ATF2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding pro
数据库入口号Swiss Prot:P15336
GeneID:1386
Uniprot
P15336
计算分子量55 kDa
实际分子量65,75 kDa
浓度1 mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存-20˚C/1
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IP 2-5 ug/mg lysate; ELISA 1:20000; IF 1:50-200
All lanes : ATF-2 (Phospho-Thr69) Antibody at 1/500 dilution Lane 1 : LOVO whole cell lysates Lane 2 : LOVO treated with Lambda Protein Phosphatase for 1 hour whole cell Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 65,75 kDa Exposure time: 7 seconds
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 (Phospho-Thr69) Antibody #14065. The picture on the right is treated with the synthesized peptide.
activating transcription factor 2(ATF2) Homo sapiens This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014
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et al,ACSS2 acts as a lactyl-CoA synthetase and couples KAT2A to function as a lactyltransferase for histone lactylation and tumor immune evasion
, (2025),
PMID:
39561764
Yes
