产品详情
产品名称20S Proteasome α3 (Phospho-Ser250) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB;IHC;IF;ELISA
种属反应性Human;Mouse;Rat
特异性Phospho-20S Proteasome α3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome α3 protein only when phosphorylated at S250.
免疫原描述The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250. AA range:206-255
标记Unconjugated
别名PSMA3; HC8; PSC8; Proteasome subunit alpha type-3; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8
数据库入口号Swiss Prot:P25788
GeneID:5684
Uniprot
P25788
计算分子量32 kDa
实际分子量32 kDa
浓度1 mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存-20˚C/1
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
All lanes : 20S Proteasome α3 (Phospho-Ser250) Antibody at 1/500 dilution Lane 1 : 3T3 whole cell lysates Lane 2 : 3T3 treated with 200ng/ml EGF for 30 minutes whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 4 seconds
Immunohistochemical analysis of paraffin-embedded human brain tissue using 20S Proteasome α3 (Phospho-Ser250) Antibody #14120. The picture on the right is treated with the synthesized peptide.
proteasome subunit alpha 3(PSMA3) Homo sapiens The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
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et al,Sensory neuron TRPV1-mediated macrophage polarization and immune response regulate dental implant osseointegration
, (2025),
PMID:
41297248
Yes
