Yes
All lanes : FGFR3 (Phospho-Tyr724) Rabbit mAb at 1/1k dilution
Lane 1 :MCF7 whole cell lysates
Lane 2 :MCF7 treated with pervanadate cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 5 seconds
Immunocytochemistry/Immunofluorescence FGFR3 (Phospho-Tyr724) antibody (14262)
ICC/IF staining of FGFR3 (Phospho-Tyr724) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14262 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
