Yes
All lanes : Tau (Phospho-Ser404) Rabbit mAb at 1/1k dilutionLane 1 : Mouse kidney lysateLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 50-70 kDa Observed band size: 55 kDaExposure time: 8 seconds
All lanes : Tau (Phospho-Ser404) Rabbit mAb at 1/1k dilutionLane 1 : Mouse cerebellum lysateLane 2 :Mouse hippocampus LysatesLane 3 :Rat cerebellum lysateLane 4 :Rat hippocampus Lysatesproteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 50-70 kDa Observed band size: 55 kDaExposure time: 12 seconds
Formalin-fixed;paraffin-embedded mouse brain tissue stained for Tau (Phospho-Ser404) using 14288 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Tau (Phospho-Ser404) antibody (14288)
ICC/IF staining of Tau (Phospho-Ser404) in SH-SY5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14288 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
