产品详情
产品名称GSK3 alpha Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total GSK3a protein. and does not detect GSK-3b protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.19~23 (T-S-S-F-A) derived from Human GSK3a.
基因/蛋白名称GSK3a
标记Unconjugated
别名Factor A, GSK-3 alpha, kinase GSK3-alpha,
数据库入口号Swiss-Prot: P49840
NCBI Protein: NP_063937.2
Uniprot
P49840
计算分子量51kDa
实际分子量51kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-2000; IHC 1:50-300; IF 1:50-300;
Western Blot analysis of extracts from various cell lines using GSK3 alpha Antibody.
Western Blot analysis of extracts from 293 cells using GSK3 alpha Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 alpha Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using GSK3 alpha Antibody.
Immunofluorescence staining of methanol-fixed MCF7 cells using GSK3 alpha Antibody(red).The nuclear counter stain is DAPI (blue).
Immunofluorescence staining of methanol-fixed Hela cells using GSK3 alpha Antibody.
Immunofluorescence analysis of NIH/3T3 cells using GSK3 alpha Antibody(red). Blue: DAPI for nuclear staining.
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Cross D A, et al. (1995) Nature. 378: 785-789.
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et al,Cleavage of GSK-3尾 by Calpain Counteracts the Inhibitory Effect of Ser9 Phosphorylation on GSK-3尾 Activity Induced by H鈧侽锟?In J Neurochem on 2013 Jul by Ye Feng, Yiyuan Xia,et al..PMID:23646926
, (2013),
PMID:
23646926
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