产品详情
产品名称HER2/ErbB2 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total HER2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2.
基因/蛋白名称HER2
标记Unconjugated
别名C-erbB-2; ErbB2;
数据库入口号Swiss-Prot: P04626
NCBI Protein: NP_001005862.1
Uniprot
P04626
计算分子量138kDa
实际分子量185kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-2000; IHC 1:50-200; IF 1:50-200;
Western blot analysis of extracts from MCF and SKOV3 cells using HER2/ErbB2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human breast carcinoma using HER2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using HER2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using HER2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Rat testis tissue using HER2 Polyclonal Antibody.
Immunofluorescence analysis of MCF7 cells using HER2 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human breast carcinoma using HER2 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HEK293 cells using HER2 Polyclonal Antibody. Picture A:Target (red). Picture B: DAPI (blue). Picture C: Merge of A+B
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002)Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.
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et al,The 鈥淗ER2鈥揚I3K/Akt鈥揊ASN Axis锟?Regulated Malignant Phenotype of Colorectal Cancer Cells. In Lipids on 2012 Apr by Nan Li, Xiaodong Bu, et al..PMID:22218925
, (2012),
PMID:
22218925
et al,Loss of Fatty Acid Synthase Inhibits the "HER2-PI3K/Akt Axis" Activity and Malignant Phenotype of Caco-2 Cells.In Lipids Health Dis on 2013 Jun 1 by Nan Li, Heng Lu,et al..PMID:23725225
, (2013),
PMID:
23725225
et al,Fatty Acid Synthase Regulates Proliferation and Migration of Colorectal Cancer Cells via HER2-PI3K/Akt Signaling Pathway.In Nutr Cancer on 2012 Aug by Nan Li, Xiaodong Bu,et al..PMID:22860766
, (2012),
PMID:
22860766
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