产品详情
产品名称Rb Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Rb protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.
基因/蛋白名称Rb
标记Unconjugated
别名P105-RB; PP105; PP110; RB-1; RB1
数据库入口号Swiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 cells using Rb(Ab-807) Antibody #21109.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Ab-807) Antibody #21109(left) or the same antibody preincubated with blocking peptide(right).
Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter
Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
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