产品详情
产品名称Smad2 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB;IHC;IF;CoIP;ELISA
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total Smad2 protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.
基因/蛋白名称Smad2
标记Unconjugated
别名JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
数据库入口号Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1
Uniprot
Q15796
计算分子量52kDa
实际分子量52-60kDa
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000-1:20000; CoIP 1:25-200
Western blot analysis of extracts from 293 and NIH-3T3 cells using Smad2 Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using Smad2 Polyclonal Antibody(left) or the same antibody preincubated with blocking peptide(right).
Immunohistochemistry of paraffin-embedded Human urothelial carcinoma tissue using Smad2 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human tonsil tissue using Smad2 Polyclonal Antibody.
Immunofluorescence staining of methanol-fixed Hela cells using Smad2 Polyclonal Antibody.
Immunofluorescence staining of methanol-fixed Jurkat cells using Smad2 Polyclonal Antibody.
Immunofluorescence staining of methanol-fixed HepG2 cells using Smad2 Polyclonal Antibody.
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420
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