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位置: 首页 > 多克隆抗体 > OCT4/POU5F1 Polyclonal Antibody

OCT4/POU5F1 Polyclonal Antibody#21424

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产品详情

产品名称OCT4/POU5F1 Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB;IHC;ELISA

种属反应性Human;Mouse

特异性The antibody detects endogenous level of total OCT-4 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4

基因/蛋白名称OCT-4

标记Unconjugated

别名Otc3; OTF3; OTF4; OTF-3;

数据库入口号Swiss-Prot: Q01860
NCBI Protein: NP_002692.2

Uniprot Q01860

计算分子量39kDa

实际分子量50kDa

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
WB 1:2000-1:5000;IHC 1:500-1:2000;
Western blot analysis of extracts from K562 cells using OCT4/POU5F1 Polyclonal Antibody.

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278.
Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
 

如果您使用该产品21424发表了文章,请通知我们,让我们可以引用您的文献。

et al,Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics.In Mol Med Rep on 2020 May;21 by Wang B, Wang L, et al..PMID: 32186775 , (2020),
PMID: 32186775
et al,Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway.In Mol Med Rep.On 2016 Oct by Gu H, Ji R et al..PMID:27513187 , (2016),
PMID: 27513187
et al,Exosomal TRIM3 is a novel marker and therapy target for gastric cancer.In J Exp Clin Cancer Res.On 2018 Jul 21 by Fu H, Yang H et al..PMID:30031392 , (2018),
PMID: 30031392
et al,H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells.In Int J Mol Med on 2013 Dec by Zhang Q, Wang M,et al..PMID:24145921 , (2013),
PMID: 24145921
et al,Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness.In BMC Cancer on 2015 Oct 24 by Jianguo Xue , Yuan Zhu et al..PMID: 26498753 , (2015),
PMID: 26498753
et al,Activation of mesenchymal stem cells by macrophages prompts human gastric cancer growth through NF-魏B pathway.In PLoS One on 2014 May 13 by Tingting Yang , Xu Zhang et al..PMID:24824968 , (2014),
PMID: 24824968

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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