产品详情
产品名称ribosome binding protein 1 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Purified by antigen-affinity chromatography.
应用WB IHC IF
种属反应性Human
免疫原类型Recombinant protein
免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 750 and 957 of ribosome binding protein 1
基因/蛋白名称ribosome binding protein 1
标记Unconjugated
数据库入口号NCBI Gene ID: 6238
NCBI mRNA#: NM_004587
NCBI Protein#: NP_004578
浓度1mg/ml
配方Supplied in 1XPBS, 40% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
应用详情
Predicted MW: 109kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1 antibody at 1: 500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2500.
Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
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