产品详情
产品名称NDUFB5 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Purified by antigen-affinity chromatography.
应用WB IHC IF
种属反应性Human
免疫原类型Peptide
免疫原描述Synthetic peptide contain a sequence corresponding to a region within amino acids 126 and 189 of human NDUFB5
基因/蛋白名称NDUFB5
标记Unconjugated
数据库入口号NCBI Gene ID: 4711
NCBI mRNA#: NM_002492
NCBI Protein#: NP_002483
浓度0.4mg/ml
配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
应用详情
Predicted MW: 22kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
Primary antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5 antibody at 1: 500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5 antibody at 1: 200 dilution.
The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]
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et al,analysis: an analysis based on the European spontaneous adverse event reporting system
, (2025),
PMID:
39877384
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