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位置: 首页 > 鼠单克隆抗体 > WDR77 Monoclonal Antibody

WDR77 Monoclonal Antibody #27213

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产品详情

产品名称WDR77 Monoclonal Antibody

克隆号8A10-C10-E8

来源种属Mouse

克隆性Monoclonal

亚型IgG1

纯化Affinity purified

应用WB ICC

种属反应性Human;Mouse;Rat

特异性This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.

免疫原类型Recombinant Protein

免疫原描述Purified recombinant human WDR77 protein fragments expressed in E.coli.

基因/蛋白名称WDR77

标记Unconjugated

别名2610312E17Rik; Androgen receptor cofactor p44; C79984; HKMT1069; MEP 50; MEP-50; MEP50; MEP50_HUMAN; Methylosome protein 50; MGC2722; Nbla10071; p44; p44/Mep50; RGD1310479; RP11 552M11.3; WD repeat containing protein 77; WD repeat domain 77;

数据库入口号Uniprot: Q9BQA1

Gene ID: 79084

Uniprot Q9BQA1

实际分子量42kd

配方Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

保存store at -20˚C

应用详情

Western blotting: 1:1000

Immunocytochemistry: 1:100
Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using anti-WDR77 antibody (dilution 1:100).
Western blot detection of WDR77 antibody in C6,3T3 and K562 cell lysates using WDR77 antibody (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

 Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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