产品详情
产品名称MCM3 Antibody
来源种属Rabbit
克隆性Polyclonal
应用ELISA WB IHC
种属反应性Human
特异性The antibody detects endogenous level of total MCM3 protein.
免疫原类型Recombinant protein
免疫原描述Fusion protein corresponding to C terminal 300 amino acids of human minichromosome maintenance complex component 3
基因/蛋白名称MCM3
标记Unconjugated
别名minichromosome maintenance complex component 3, HCC5, P1.h, RLFB, P1-MCM3
数据库入口号Genbank No.: BC001626
Uniprot
BC001626
配方Supplied at 0.8mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
保存Store at -20˚C/1 year
应用详情
Predicted MW: 91kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Gel: 8%SDS-PAGE
Lane1: K562 cell lysate
Lane2: Jurkat cell lysate
Lane3: Hela cell lysate
Lane4: 293T cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/550 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using 31099 (MCM3 Antibody) at dilution 1/30, on the right is treated with the fusion protein.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
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et al,ACSS2 acts as a lactyl-CoA synthetase and couples KAT2A to function as a lactyltransferase for histone lactylation and tumor immune evasion
, (2025),
PMID:
39561764
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