产品详情
产品名称LDHA Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total LDHA protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human LDHA .
基因/蛋白名称LDHA
标记Unconjugated
别名LDHA; LDH-M; LDH1; PIG19;
数据库入口号Swiss-Prot:P00338
NCBI Gene ID:3939
Uniprot
P00338
实际分子量37KD
浓度1.0mg/ml
配方PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH7.3.
保存Store at -20˚C
应用详情
WB:1:500 - 1:2000;
IHC:1:50 - 1:200;
IF:1:50 - 1:200
Western blot analysis of lysates from HeLa cells, using LDHA Antibody at 1:2000 dilution.
Western blot analysis of various lysates, using LDHA Antibody at 1:1000 dilution.
Western blot analysis of lysates from Rat liver using LDHA Antibody at 1:800 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LDHA Antibody at a dilution of 1:100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using LDHA Antibody at a dilution of 1:100 (40x lens).
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using LDHA Antibody at a dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using LDHA Antibody at dilution of 1:50 (40x lens).
Immunofluorescence analysis of MCF7 cells using LDHA Antibody at dilution of 1:50 (40x lens).
Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.
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et al,Dendrobium officinale polysaccharide inhibits M1 macrophage polarization via activating SENP1-SIRT3 signaling and alleviates ulcerative colitis
, (2025),
PMID:
41185333
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