有货
Western blot analysis of various lysates using CHRNA1 Antibody at 1:1000 dilution;
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Detection: ECL Basic Kit;
Exposure time: 90s
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution;
Lysates/proteins: 25 ug per lane;
Blocking buffer: 3% nonfat dry milk in TBST;
Detection: ECL Basic Kit;
Exposure time: 90s
Immunohistochemistry analysis of paraffinembedded Human skeletal muscle tissue using CHRNA1 Antibody at a dilution of 1:100 (40X lens);
High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffinembedded Mouse heart using CHRNA1 Antibody at a dilution of 1:100 (40X lens);
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining;
Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution;
Blue: DAPI for nuclear staining;
Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
