产品详情
产品名称PDGFR α Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous levels of total PDGFR α protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from internal of human PDGFR α.
基因/蛋白名称PDGFR α
标记Unconjugated
别名Alpha platelet-derived growth factor receptor precursor; CD140a; PDGF-R-alpha; PDGFR-alpha; PGFRA
数据库入口号Swiss-Prot: P16234
NCBI Gene ID: 5156
Uniprot
P16234
实际分子量140kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDGFR α antibody #33470.
Immunofluorescence analysis of HeLa cells, using PDGFR α antibody #33470.
Western blot analysis of extracts from HepG2 cells, using PDGFR α antibody #33470.
Western blot analysis of extracts from 293 cells (Lane 2), using PDGFR α antiobdy #33470. The lane on the left is treated with synthesized peptide.
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
Jennifer Brennan, Genes & Dev., Mar 2003; 17: 800.
JC Yu, J. Biol. Chem., Apr 1994; 269: 10668 - 10674.
Nobuyuki Takakura, J. Histochem. Cytochem., Jun 1997; 45: 883.
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et al,Cancer-associated fibroblasts promote lymphatic metastasis in cholangiocarcinoma via the PDGF-BB/PDGFR-β mediated paracrine signaling network. In Aging Dis on 2024 Feb 1 by Jian Yan, Gang Xiao,et al..PMID:37307823
, (2024),
PMID:
37307823
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