产品详情
产品名称MASTL Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB
种属反应性Human
特异性The antibody detects endogenous levels of total MASTL protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from C-terminal of human MASTL.
基因/蛋白名称MASTL
标记Unconjugated
别名 FLJ14813; MASTL; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2
数据库入口号Swiss-Prot: Q96GX5
NCBI Gene ID: 84930
Uniprot
Q96GX5
实际分子量97kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Western blot analysis of extracts from HeLa cells, using MASTL antibody #35289.
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Ota T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 429:375-381(2004).
Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).
如果您使用该产品35289发表了文章，请通知我们，让我们可以引用您的文献。
et al,ERK–USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth
, (2025),
PMID:
39824978
有货
