产品详情
产品名称ILF3 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total ILF3 protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human ILF3.
基因/蛋白名称ILF3
标记Unconjugated
别名CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90;
数据库入口号Swiss-Prot#: Q12906
NCBI Gene ID: 3609
Uniprot
Q12906
实际分子量95kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500 - 1:2000
Western blot analysis of extracts of various cell lines, using ILF3 antibody at 1:1000 dilution.
Western blot analysis of extracts of mouse thymus, using ILF3 antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ILF3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]
如果您使用该产品38409发表了文章，请通知我们，让我们可以引用您的文献。
et al,PI3Kβ functions as a protein kinase to promote cellular protein O-GlcNAcylation and acetyl-CoA production for tumor growth
, (2025),
PMID:
40132583
Yes
