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RIPK3 antibody#38654

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产品详情

产品名称RIPK3 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were purified by affinity purification using immunogen.

应用WB

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of total RIPK3 protein.

免疫原类型Recombinant Protein

免疫原描述Recombinant protein of Human RIPK3.

基因/蛋白名称RIPK3

标记Unconjugated

别名RIP3;

数据库入口号Swiss-Prot#: Q9Y572
NCBI Gene ID: 11035

Uniprot Q9Y572

实际分子量56kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:2000

Western blot analysis of extracts of various cell lines, using RIP3 antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using RIP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using RIP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human liver cancer using RIP3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using RIP3 antibody at dilution of 1:100 (40x lens).

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

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et al,Yes-associated protein inhibition ameliorates liver fibrosis and acute and chronic liver failure by decreasing ferroptosis and necroptosisInHeliyonOn?2023 Apr 3byWen Zhao?1?2?3,?Miao Lei et al..PMID:?37151632 , (2023),
PMID: 37151632

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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