产品详情
产品名称Cleaved-Caspase-9 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB;IHC;IF
种属反应性Human;Mouse;Rat
特异性Cleaved-Caspase-9 p10 Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-9 p10 protein resulting from cleavage adjacent to D330.
免疫原类型Peptide
免疫原描述The antiserum was produced against synthesized peptide derived from human Caspase 9.
基因/蛋白名称Cleaved-Caspase-9
标记Unconjugated
别名CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
数据库入口号P55211
Uniprot
P55211
Gene ID
842
实际分子量10kDa,46kDa
浓度1mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20˚C/1 year
应用详情
WB 1:500-1:2000;
IHC 1:100-1:300;
IF 1:50-1:200
Western Blot analysis of extracts from HEPG2-UV cells using Cleaved-Caspase-9 Polyclonal Antibody
Western Blot analysis of extracts from NIH-3T3 and HepG2 cells using Cleaved-Caspase-9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using Cleaved-Caspase-9 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using Cleaved-Caspase-9 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using Cleaved-Caspase-9 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using Cleaved-Caspase-9 Polyclonal Antibody.
Immunofluorescence analysis of human stomach cancer tissue using CASP9 antibody .
Immunofluorescence analysis of HepG2 cells using CASP9 antibody .
Immunofluorescence analysis of MCF-7 cells using CASP9 antibody .
Western Blot analysis of various cells using Cleaved-Caspase-9 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of various cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:10000
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue;
1.Cleaved-Caspase-9 Polyclonal Antibody was diluted at 1:200(4℃,overnight);
2.Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min);
3.Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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