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PI3 Kinase p85 alpha Polyclonal Antibody#41339

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产品详情 图片 产品描述 发表文献(5) 其它
产品详情

产品名称PI3 Kinase p85 alpha Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC IF ELISA

种属反应性Human;Mouse;Rat

特异性PI 3-kinase p85α Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein.

免疫原类型peptide

免疫原描述Synthesized peptide derived from human PI 3-kinase p85α around the non-phosphorylation site of Y607.

基因/蛋白名称PI 3-kinase p85α

标记Unconjugated

别名PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph

数据库入口号Swiss-Prot: P27986
NCBI Gene ID: 5295

Uniprot P27986

计算分子量85kDa

实际分子量85kDa

浓度1mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C/1 year

应用详情
Western Blot: 1:500-2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/5000
Western blot analysis of extracts from various cell lines using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.
Immunofluorescence analysis of PC-12 cells using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PI3 Kinase p85 alpha Rabbit Polyclonal Antibody.Blue: DAPI for nuclear staining.

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et al,Inhibition of miRNA?21 attenuates the proliferation and metastasis of human osteosarcoma by upregulating PTEN.In Exp Ther Med.On 2018 Jan by Li C, Xu B et al..PMID:29434694 , (2018),
PMID: 29434694
et al,IvabradIne improved left ventricular function and pressure overload-induced cardiomyocyte apoptosis in a transverse aortic constriction mouse model. In Mol Cell Biochem on 2019 Jan by Yu Y, Hu Z, et al..PMID:29790114 , (2019),
PMID: 29790114
et al,Neferine ameliorates hypertensive vascular remodeling modulating multiple signaling pathways in spontaneously hypertensive ratsInBiomed PharmacotherOn2023 FebbyWeiquan Zeng?1,?Xiuli Zhang et al..PMID:?36916429 , (2023),
PMID: 36916429
et al,Alpha-lipoic acid alleviates NAFLD and triglyceride accumulation in liver via modulating hepatic NLRP3 inflammasome activation pathway in type 2 diabetic rats. In Food Sci Nutr on 2021 Mar 13 by Chih-Yuan Ko, Yangming Martin Lo, et al..PMID: 34026086 , (2021),
PMID: 34026086
et al,Cardioprotection of ( )-sodium 5-bromo-2-(α-hydroxypentyl) benzoate (BZP) on mouse myocardium I/R injury through inhibiting 12/15-LOX-2 activity. In J Mol Cell Cardiol on 2019 Oct by Xiao Y, Song C, et al..PMID:31362020 , (2019),
PMID: 31362020

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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