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Seprase Polyclonal Antibody #41425

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产品详情

产品名称Seprase Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB;IHC;IF

种属反应性Human;Mouse;Rat

特异性Seprase Polyclonal Antibody detects endogenous levels of Seprase protein.

免疫原描述The antiserum was produced against synthesized peptide derived from human FAP-1

基因/蛋白名称FAP

标记Unconjugated

别名FAP; Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease

数据库入口号Swiss-Prot: Q12884
NCBI Gene ID: 2191

Uniprot Q12884

实际分子量90kd

浓度1mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C/1 year

应用详情
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:100-1:300
All lanes : Fibroblast activation protein, alpha Antibody at 1/500 dilution
Lane 1 : LOVO whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : Mouse kidney lysates
Lane 5 : Rat muscle lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 90 kDa
Exposure time: 8 seconds
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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et al,Ultrasensitive fluorescent probes reveal an adverse action of dipeptide peptidase IV and fibroblast activation protein during proliferation of cancer cells.In Anal Chem on 2016 Aug 16 by Qiuyu Gong, Wen Shi et al..PMID:27444320 , (2016),
PMID: 27444320

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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