产品详情
产品名称ETFB Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Human;Mouse
特异性The antibody detects endogenous levels of total ETFB protein.
免疫原类型peptide
免疫原描述Synthetic peptide of human ETFB
基因/蛋白名称ETFB
标记Unconjugated
别名MADD; FP585
数据库入口号Swiss-Prot#: P38117
NCBI Gene ID: 2109
Uniprot
P38117
计算分子量28kd
浓度0.4mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:200-1000
Immunohistochemistry: 1: 20-100
Gel: 12%SDS-PAGE
Lysate: 40 μg, Lane 1-6: HEPG2 and Hela cell lysates£¬Human heart tissue£¬mouse brain tissue£¬mouse skeletal muscle tissue£¬human liver tissue lysates,
Primary antibody:ETFB antibody at dilution 1/200,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ETFB Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.
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et al,Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. In Cell Mol Life Sci on 2022 Jun 21 by Min-Zhi Peng, Yong-Xian Shao, et al..PMID:35727412
, (2022),
PMID:
35727412