产品详情
产品名称GAPDH Antibody
克隆号5-E10
来源种属Mouse
克隆性Monoclonal
纯化ProL affinity purified
应用WB, ICC
种属反应性Hu,Ms,Rt,Zebrafish,rabbit
免疫原描述peptide
标记Unconjugated
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数据库入口号Swiss-Prot#:P04406
Uniprot
P04406
计算分子量36 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:5,000-1:10,000
ICC: 1:200
Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution.
Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: MCF-7 Lane 6: Rabbit liver Lane 7:Zebrafish
ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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et al,The bone marrow immune ecosystem shapes daratumumab acquired resistance in plasma cell myeloma: MULTIPLE MYELOMA, GAMMOPATHIES
, (2025),
PMID:
40750676
Yes
