产品详情
产品名称Cdk9 Rabbit mAb
克隆号SD204-07
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名C-2K antibody CDC2 related kinase antibody CDC2L4 antibody Cdk 9 antibody Cdk9 antibody CDK9_HUMAN antibody Cell division cycle 2-like protein kinase 4 antibody Cell division protein kinase 9 antibody CTK1 antibody Cyclin dependent kinase 9 antibody Cyclin-dependent kinase 9 antibody PITALRE antibody Serine/threonine-protein kinase PITALRE antibody TAK antibody Tat associated kinase complex catalytic subunit antibody
数据库入口号Swiss-Prot#:P50750
Uniprot
P50750
计算分子量43/37 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Cdk9 on different lysates using anti-Cdk9 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: A431
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
ICC staining Cdk9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1�C9, PISSLRE, KKIALRE, PITSLRE, and PCTAIRE 1�C3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1�C3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.
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