产品详情
产品名称ATF1 Rabbit mAb
克隆号JJ207-04
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名Activating transcription factor 1 antibody ATF 1 antibody atf1 antibody ATF1 EWS fusion gene antibody ATF1 FUS fusion gene antibody ATF1_HUMAN antibody cAMP dependent transcription factor 1 antibody cAMP-dependent transcription factor ATF-1 antibody Cyclic AMP dependent transcription factor ATF 1 antibody Cyclic AMP dependent transcription factor ATF1 antibody Cyclic AMP-dependent transcription factor ATF-1 antibody EWS AFT1 antibody FUS ATF 1 antibody FUS/ATF 1 antibody Protein TREB36 antibody RNA binding protein activating transcription factor 1 fusion protein antibody TREB 36 antibody TREB36 antibody TREB36 protein antibody
数据库入口号Swiss-Prot#:P18846
Uniprot
P18846
计算分子量29 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1;500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF1 antibody. Counter stained with hematoxylin.
ICC staining ATF1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ATF1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
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