产品详情
产品名称Caspase-10 Rabbit mAb
克隆号JJ0890
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, IHC, IP, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名ALPS2 antibody Apoptotic protease Mch-4 antibody CASP 10 antibody CASP-10 antibody CASP10 antibody CASPA_HUMAN antibody Caspase 10 apoptosis related cysteine peptidase antibody Caspase-10 subunit p12 antibody FADD like ICE2 antibody Fas associated death domain protein antibody FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody FLICE 2 antibody FLICE2 antibody ICE like apoptotic protease 4 antibody ICE-like apoptotic protease 4 antibody Interleukin 1B converting enzyme 2 antibody MCH 4 antibody
数据库入口号Swiss-Prot#:Q92851
Uniprot
Q92851
计算分子量59 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Caspase-10 on K562 cells lysates using anti-Caspase-10 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-10 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of K562 cells with Caspase-10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Caspase-10, also designated Mch4, is recruited to the native TRAIL and CD9 death-inducing signaling complexes (DISCs) by the FADD/Mort1 adaptor protein complex. Caspase-10 requires the assembly of the FADD and DISC complexes for its recruitment and cleavage-induced activation during CD95-induced apoptosis of activated T cells. The N-terminus of caspase-10 contains FADD-like death effector domains further indicating that it associates with FADD to induce apoptosis. Caspase-10 is not required for apoptosis induction and when overexpressed, cannot reverse defects in apoptosis induction caused by caspase-8 deficiency. Granzyme B cleaves procaspase-10 at an IXXD-A processing sequence to produce mature caspase-10. Mutations in the caspase-10 gene in the prodomain, p17 large protease subunit and p12 small protease subunit have been linked to a number of non-Hodgkin lymphomas in humans.
如果您使用该产品49264发表了文章，请通知我们，让我们可以引用您的文献。
Yes
