产品详情
产品名称COX IV Rabbit mAb
克隆号JJ09-05
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名AL024441 antibody COX 4 antibody COX IV 1 antibody COX IV antibody COX IV-1 antibody Cox4 antibody COX41_HUMAN antibody Cox4a antibody COX4B antibody COX4I1 antibody COX4I2 antibody COX4L2 antibody COXIV antibody Cytochrome c oxidase polypeptide IV antibody Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody Cytochrome C Oxidase subunit IV antibody Cytochrome c oxidase subunit IV isoform 1 antibody Cytochrome c oxidase subunit IV isoform 2 (lung) antibody Cytochrome c oxydase subunit 4 antibody dJ857M17.2 antibody MGC105470 antibody MGC72016 antibody
数据库入口号Swiss-Prot#:P13073
Uniprot
P13073
计算分子量20 kDa
实际分子量17 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-COX IV antibody. Counter stained with hematoxylin.
ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining COX IV in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with COX IV antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: COX IV Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : 293 whole cell lysates Lane 2 : MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 20 kDa Observed band size: 17 kDaExposure time: 7 seconds
All lanes: COX IV Rabbit mAb at 1/1k dilutionLane 1 : Mouse kidney lysates Lane 2 : Mouse brain lysates Lane 3 : Rat kidney lysates Lane 4 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 20 kDa Observed band size: 17 kDaExposure time: 9 seconds
All lanes:COX IV Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HT-1080 cell lysateLane 2 :COX IV Rabbit mAb knockdown HT-1080 cell lysateLysates/proteins at 20 µg per lane.
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.
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