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位置: 首页 > 重组兔单抗 > COX IV Rabbit mAb

COX IV Rabbit mAb #49275

是否有货: Yes
产品详情

产品名称COX IV Rabbit mAb

克隆号JJ09-05

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名AL024441 antibody
COX 4 antibody
COX IV 1 antibody
COX IV antibody
COX IV-1 antibody
Cox4 antibody
COX41_HUMAN antibody
Cox4a antibody
COX4B antibody
COX4I1 antibody
COX4I2 antibody
COX4L2 antibody
COXIV antibody
Cytochrome c oxidase polypeptide IV antibody
Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
Cytochrome C Oxidase subunit IV antibody
Cytochrome c oxidase subunit IV isoform 1 antibody
Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
Cytochrome c oxydase subunit 4 antibody
dJ857M17.2 antibody
MGC105470 antibody
MGC72016 antibody

数据库入口号Swiss-Prot#:P13073

Uniprot P13073

计算分子量20 kDa

实际分子量17 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-COX IV antibody. Counter stained with hematoxylin.
ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining COX IV in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with COX IV antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: COX IV Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysates
Lane 2 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 7 seconds
All lanes: COX IV Rabbit mAb at 1/1k dilution
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat kidney lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 9 seconds
All lanes:COX IV Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 :COX IV Rabbit mAb knockdown HT-1080 cell lysate
Lysates/proteins at 20 µg per lane.
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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