产品详情
产品名称HDAC6 Rabbit mAb
克隆号JJ09-09
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CPBHM antibody FLJ16239 antibody HD 6 antibody HD6 antibody HDAC 6 antibody HDAC6 antibody HDAC6_HUMAN antibody Histone deacetylase 6 (HD6) antibody Histone deacetylase 6 antibody JM 21 antibody JM21 antibody KIAA0901 antibody OTTHUMP00000032398 antibody OTTHUMP00000197663 antibody PPP1R90 antibody Protein phosphatase 1 regulatory subunit 90 antibody
数据库入口号Swiss-Prot#:Q9UBN7
Uniprot
Q9UBN7
计算分子量131 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of HDAC6 on Jurkat cells lysates using anti-HDAC6 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC6 antibody. Counter stained with hematoxylin.
ICC staining HDAC6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HDAC6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HDAC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.
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